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KMID : 0615720170340020083
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Jouranl of Korean Acupuncture and Moxibustion society
2017 Volume.34 No. 2 p.83 ~ p.91
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Anti-inflammatory and Anti-oxidative Effects of Alpiniae Oxyphyllae Fructus Hot Aqueous Extract in Lipopolysaccharide (LPS)-stimulated Macrophages
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Jo Na-Young
Lee Pyeong-Jae
Roh Jeong-Du
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Abstract
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Objectives: Alpiniae oxyphyllae Fructus (AOF) is an herbal medicine, which has been used for the treatment of fatigue, chills, and poor physical conditions. The objective of this study was to investigate the anti-inflammatory and anti-oxidative effects of AOF hot aqueous extract.
Methods: The cytotoxicity of AOF extract was evaluated using the MTT assay. Nitric oxide (NO) production was measured by the Griess reaction. Prostaglandin E2 (PGE2) production was measured by a commercial competitive enzyme immunoassay. Cytokine production (IL - 1tion co6, and TNF-F- was measured by ELISA. The anti-oxidative effect of AOF extracts was measured by the DPPH method. Polyphenol and flavonoid contents were measured by Folin-Ciocalteu’s phenol reagent and aluminum chloride, respectively.
Results: AOF hot aqueous extract did not show toxicity at doses of 25, 50, 100, and 200 μg/mL. AOF extract significantly inhibited NO production at doses of 100 and 200 μg/mL. PGE2 production was inhibited by AOF extract treatment at doses of 100 and 200 μg/mL. AOF extracts reduced IL-6 production in a dose-dependent manner. IL-1ent maTNF-F-1ent mannerd IL-6 production in uction at doses of 100 and μg/mL. The DPPH free radical scavenging capability was above 50% at 200 μg/mL.
Conclusion: This study suggests that AOF hot aqueous extract may exert anti-inflammatory and anti-oxidative effects in a dose-dependent manner. Further studies are required for validating the safety and efficacy of AOF.
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KEYWORD
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Alpiniae oxyphyllae Fructus, Anti-inflammation, Antioxidant, Hot Aqueous Extract, Korean Medicine
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